used for reduction, but with less success; Stedman, unpublished).
The air in the tube is displaced with CO2 and N2 by the
Hungate technique and the tube is stoppered (Hungate et al.
1966). A cap is placed on the tube and the assemblage autoclaved.
A gas phase of 160 kPa of CO2 and 1 kPa of H2S has also
been used successfully (Prangishvili 2006).
Sample collection and transportation for thermoacidophilic
Archaea—Liquid and wet sediment samples are collected from
turbid terrestrial hot springs with high temperature >70°C and
low pH