A reversed-phase HiCHROM LiChrosorb RP8-10 C18
column (250 mm4.6 mm I.D., particle size 5 mm) was
used for separation at ambient temperature (2570.5 1C).
The column was equilibrated with the mobile phase (flow rate
1.0 mL/min); methanol/water/acetonitril (25/65/10, v/v). The
injection volume was 25 mL, and the detection wavelength was
240 nm.