The process of physiological ageing in woody plants is a very important factor influencing adventitious
rooting. However, there is a lack of knowledge of biochemical backgrounds triggering ageing and consequently,
rhizogenesis. Experiments with Prunus subhirtella ‘Autumnalis’ leafy cuttings of three different
physiological ages (adult (over 40-year-old stock plants), semi-adult (5-year-old cutting plants) and juvenile
(5-year-old in vitro plants)) were conducted in 2009. Half of the cuttings were banded ca. 3 cm above
the bottom of the cutting with aluminum wire prior to insertion into the substrate to block the polar
auxin transport. IBA, which was exogenously applied to the cuttings, could only be detected in the base
of the cuttings on the first day after severance. Juvenile cuttings tended to have the highest values, but
the effect was age specific. Later, the detection was not possible, regardless of the age. The IAA profile
in cutting bases was similar for all physiological ages, reaching the peak on the first day after severance.
Juvenile cuttings, in which the stems had been banded before insertion, contained more IAA in their
bases on day 1 compared to the stems, which were not banded. These cuttings presumably transported
absorbed auxin mainly via phloem, and not via mass flow like semi-adult and adult cuttings, where IAA
concentrations were similar or even greater in non-banded cuttings compared to banded ones. These
cuttings also tended to exhibit the best rooting results. The IAA-Asp accumulation was especially strong
in adult cuttings, which contained significantly more aspartate on the first and third days after severance
when compared with semi-adult and juvenile cuttings.
The process of physiological ageing in woody plants is a very important factor influencing adventitiousrooting. However, there is a lack of knowledge of biochemical backgrounds triggering ageing and consequently,rhizogenesis. Experiments with Prunus subhirtella ‘Autumnalis’ leafy cuttings of three differentphysiological ages (adult (over 40-year-old stock plants), semi-adult (5-year-old cutting plants) and juvenile(5-year-old in vitro plants)) were conducted in 2009. Half of the cuttings were banded ca. 3 cm abovethe bottom of the cutting with aluminum wire prior to insertion into the substrate to block the polarauxin transport. IBA, which was exogenously applied to the cuttings, could only be detected in the baseof the cuttings on the first day after severance. Juvenile cuttings tended to have the highest values, butthe effect was age specific. Later, the detection was not possible, regardless of the age. The IAA profilein cutting bases was similar for all physiological ages, reaching the peak on the first day after severance.Juvenile cuttings, in which the stems had been banded before insertion, contained more IAA in theirbases on day 1 compared to the stems, which were not banded. These cuttings presumably transportedabsorbed auxin mainly via phloem, and not via mass flow like semi-adult and adult cuttings, where IAAconcentrations were similar or even greater in non-banded cuttings compared to banded ones. Thesecuttings also tended to exhibit the best rooting results. The IAA-Asp accumulation was especially strong
in adult cuttings, which contained significantly more aspartate on the first and third days after severance
when compared with semi-adult and juvenile cuttings.
การแปล กรุณารอสักครู่..