At the same time an easy and effective method for the screening of resistance to the fungus has been developed by treating leaf discs (7 mm in diameter) by the AK-toxin produced by the fungus [25]. It was coincidental and lucky for the breeders that Nakashima, et al . [26,27] isolated and identified the chemical structure of the toxin named “AK-toxin” produced by the fungus of black spot disease and generating the symptom of black spots on leaves at the same time. As a consequence, the breeding group entered into a cooperative research programme with the chemistry group and established this unique method. When the leaf discs are placed on the filter paper soaked with AK-toxin obtained either from the extract of the fungal body or artificial synthesis in a Petri dish, and kept for two or three days, susceptible leaves turned to black and resistant leaves stayed green (Fig. 7). After the development of this method, two new mutant varieties, “Osa-Gold [28,29]” and “Kotobuki Shinsui [30]”
H NAKAGAWA
52
were developed in a short period of time by using this screening method. The economic effect of this research has been great [4]. These researches suggest that the breeding of fruit trees requires patience and that development of easy and precise screening methods is a very important addition to the development of methods for mutation induction.
At the same time an easy and effective method for the screening of resistance to the fungus has been developed by treating leaf discs (7 mm in diameter) by the AK-toxin produced by the fungus [25]. It was coincidental and lucky for the breeders that Nakashima, et al . [26,27] isolated and identified the chemical structure of the toxin named “AK-toxin” produced by the fungus of black spot disease and generating the symptom of black spots on leaves at the same time. As a consequence, the breeding group entered into a cooperative research programme with the chemistry group and established this unique method. When the leaf discs are placed on the filter paper soaked with AK-toxin obtained either from the extract of the fungal body or artificial synthesis in a Petri dish, and kept for two or three days, susceptible leaves turned to black and resistant leaves stayed green (Fig. 7). After the development of this method, two new mutant varieties, “Osa-Gold [28,29]” and “Kotobuki Shinsui [30]” H NAKAGAWA52were developed in a short period of time by using this screening method. The economic effect of this research has been great [4]. These researches suggest that the breeding of fruit trees requires patience and that development of easy and precise screening methods is a very important addition to the development of methods for mutation induction.
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