Abstract. In this study, we distinguished Cucumber green mottle mosaic virus (CGMMV) infected seeds from healthy
seeds using optical coherence tomography (OCT). Two dimensional OCT images and stereo micrographs revealed that
the infected seeds had narrow gap between the seed coat and endosperm that were not present in the healthy seeds. Three
dimensional OCT images confirmed that the narrow gaps were present in the inner structure of overall the infected
seeds. A-scan analysis was also performed to calculate the distance from the seed coat to the endosperm. The results
revealed a difference in the width of the gap of about 20 μm between healthy and infected cucumber seeds. Taken
together, these results suggest that OCT could be applied as an effective non-destructive method for CGMMV infection
of cucumber seeds.