Conventionally, cellulose film is prepared by wet extrusion process. Briefly, viscose (alkali/carbon disifide dissolved cellulose) is extruded through a slit into a bath of dilute sulfuric acid/sodium sulfate to convert the viscose into cellulose film. This process is involved with toxic chemicals. Moreover, this process is lengthy. In this work, novel method of cellulose film preparation was revealed. Alkali/urea dissolved microcrystalline cellulose in the presence of nucleation nanoparticles was prepared. The cellulose solution was cast into acrylic mold. Due to the presence of nucleation nanoparticles, fast solidification of cellulose gel film was obtained and then followed by solvent exchange with water to remove unwanted chemicals. The resultant cellulose film was treated with a solution of glycerol to prevent the film brittleness.