The filtrates were pooled and concentrated by rotary evaporator The obtained semisolid extracts were kept in a desiccator at 4°C untilfurther used.
2. Formulation of anti-acne gel from mangosteen crude extract
32 factorial design was employed for optimization. Two variables included content of
Carbopol Ultrez-10 and mangosteen crude extract. The levels of Carbopol Ultrez-10 were 0.3, 0.5
and 0.7% and mangosteen crude extract were 0.5, 0.75 and 1%. Nine Samples of anti-acne gel were
prepared.