DPPH radical scavenging ability assay
The DPPH radical scavenging ability was determined according
to the method described by Amarowicz, Karamac´ , Weidner, Abe,
and Shahidi (2002). An aliquot of 2.0 ml of 0.1 mM DPPH radical
solution was mixed with 1.0 ml of test samples at various concentrations.
Absorbance at 517 nm was measured after 10 min. BHT
was employed as positive control. The ability to scavenge DPPH
radical was calculated by the following equation: