The detailed characteristics of the isolates are
described in the species description. The biochemical
characteristics were compared with those of the type
strains of the phylogenetic relatives L. kunkeei, L. kefiri, L.
floricola and L. fructivorans (Table 1). The isolates were
heterofermentative LAB and produced D- and L-lactic acid
from D-glucose, as determined by using L- and D-lactate
dehydrogenases (Sigma) (Latorre-Guzman et al., 1977).
This finding was confirmed by performing HPLC analysis
with a separation column for optical isomers (CRS10W
column; Mitsubishi Chemical) (Otsuka et al., 1994;
Manome et al., 1998); D- and L-lactic acid were produced
at a ratio of 1 : 2. Production of ethanol from glucose was
detected by using GC. The strains grew well at 20 and 30 uC
(optimum) and grew slowly at 15 and 37 uC. The strains
produced acid from a narrow range of carbohydrates,
including glucose and fructose, and produced acid weakly
from maltose, sucrose and mannitol.
The detailed characteristics of the isolates aredescribed in the species description. The biochemicalcharacteristics were compared with those of the typestrains of the phylogenetic relatives L. kunkeei, L. kefiri, L.floricola and L. fructivorans (Table 1). The isolates wereheterofermentative LAB and produced D- and L-lactic acidfrom D-glucose, as determined by using L- and D-lactatedehydrogenases (Sigma) (Latorre-Guzman et al., 1977).This finding was confirmed by performing HPLC analysiswith a separation column for optical isomers (CRS10Wcolumn; Mitsubishi Chemical) (Otsuka et al., 1994;Manome et al., 1998); D- and L-lactic acid were producedat a ratio of 1 : 2. Production of ethanol from glucose wasdetected by using GC. The strains grew well at 20 and 30 uC(optimum) and grew slowly at 15 and 37 uC. The strainsproduced acid from a narrow range of carbohydrates,including glucose and fructose, and produced acid weaklyfrom maltose, sucrose and mannitol.
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