Strains FR95 and 99/R were grown in YPD broth (20.0 g/l peptone, 10.0 g/l yeast extract, 20.0 g/l dextrose, Oxoid) for 72 h at 30 C. Cells were collected by centrifugation at 1100 g for 10 min at 4 C and then washed in distilled water.
Strains FR95 and 99/R were grown in YPD broth (20.0 g/lpeptone, 10.0 g/l yeast extract, 20.0 g/l dextrose, Oxoid) for 72 h at30 C. Cells were collected by centrifugation at 1100 g for 10 min at4 C and then washed in distilled water.