Subsequently, a decimal dilution was made and enumeration was performed by pour-plating of 1ml appropriate sample dilutions on specific media: Plate Count Agar (PCA, Oxoid, Hampshire, U.K.) incubated at 22 ๐C for 72 h for total aerobic psychrotrophic counts, Rapid E. coli 2 Agar (Bio-Rad,Marnes-La-Coquette, France) incubated at 37 ๐C for 24 h for E. coli and coliforms and de Man Rogosa Sharpe agar (MRS, Oxoid, Hampshire, U.K.) incubated anaerobically at 22 ๐C for 72 h for lactic acid bacteria.