Yoghurt prepared by incorporation of Trapa bispinosa starch @ 0.5%, 0.75%, 1% and 1.25% was compared for these characteristics to the yoghurt containing stabilizergelatin @ 0.5% w/w. Physiochemical parameters (fat, pH, acidity, synersis, water holding capacity, viscosity, protein etc),sensory evaluation and microbial analysis (total viable count and coliform test) were studied. Use of Trapa bispinosa starchproduced better results in terms of lowering synersis and increasing water holding capacity, viscosity and overallacceptability for all sensory attributes. Addition of Trapa bispinosa starch did not influence the taste and overallacceptability. Trapa bispinosa starch @ 1.25% gave most excellent results for water holding capacity, synersis andviscosity and Trapa bispinosa starch @ 0.75% gave most excellent results for all sensory attributes. Yoghurt shelf life wasincreased upto 25 days.