The efficiency of TMC30/DNA complex was two times higher than that of both TMC60/DNA and TMC94/DNA complexes. However, it was lower compared with PEI/DNA complex as a positive control. It could be concluded that, the transfection efficiency was decreased while increasing DQ of TMC. The surface charge of polyplexes increased when using TMC with higher DQ. Having positive charge too much cause high toxicity to cells