DISCUSSION
Subject to the limitations of using a univariate experimental design and a single time port readout, lipase production by Pseudomonas aeruginosa NS2001 was found to be enhanced by optimization of the chemical (medium) as well as the physical (pH, temperature, agitation, aeration and inoculum size) parameters of the fermentation process. These partially optimized parameters in a 5-L fermenter flask resulted in a 6-fold increase in lipase production levels