The condition for microassay experiments was chosen at a virus
MOI of 5 and 24 h,based on the following results.Released viruses
were detected at 6 h postinfection and peaked at 36 h postinfection. So,24 h time point represented the active stage of infection
with increased viral titer. At this time point,we postulated that
cell state would largely represent the steady gene expression
changes. In addition, when HUVECs were infected with H9N2
viruses at a MOI of 5, we could obtain a good replication kinetic
pattern of the viruses.