Animal and fat tissues were prepared as a 2 2 design (4 systems
that contained a mixture design each). To each corner of this
2 2 design a 3-component (succinate, pyruvate and
glutamate-malate additions; 0, 50 and 100%) simplex lattice mixture
design with 2 additional centre experiments was added.
Each of these eight experimental points had 4-factors
(glutamate-malate ratio, total added mixture levels, citrate
concentrations, oxygen concentrations) at 2-levels; i.e., a 24 design.