From the HLPC analysis, the combination F. graminearum 124/rice
was chosen to further evaluate the inhibition of DON production by
T. gamsii 6085, T. gamsii 6317 and T. velutinum 4837. Twenty-five
grams of rice dipped in 12 ml distilled water in glass Petri dishes
(10 cm diameter) was autoclaved at 121 uC for 30 min. Discs of
6 mm diameter, cut from the edge of actively growing colonies on
PDA (Trichoderma) or OA (Fusarium), were placed at opposite sides
of each plate. Plates inoculated with F. graminearum 124 alone were
used as control. Three replicates for each combination were
performed. DON production [mg (kg fresh weight)21] was assessed
by HPLC, as described previously, after a 7 and a 14 day incubation
period at 24±2 uC (12 h light/12 h darkness).