Glucose concentrations were measured every 10 seconds for one minute using TRUEtest glucose metre (manufactured by Home Diagnostics, U.S.A.). The procedure was repeated for starch concentrations of 0.002, 0.003, 0.004 and
0.005 % (w/v). A Graph of glucose concentration versus time was plotted (for each starch concentration) and initial velocity of glucoamylase activity,V, determined from the equation of the curve obtained. Graphs used to determine initial velocities for glucoamylase activities obtained from Aspergillus niger and Rhizopus species are shown in Appendices 15 and 16 respectively. Hanes –Woolf’s plot (shown in Appendices13 and 14) was used to determine maximum forward velocity (Vmax) and Michaelis-Menten constant (Kmax) for glucoamylases produced by Aspergillus niger and Rhizopus species respectively.