The microculture technique reported by Tuite (1969) was employed with the following modifications: 1 cm2 agar plugs
were obtained from sterile solid medium and placed onto a slide. The slide was placed into a humidified chamber, consisting of a 9 mm-diameter Petri dish which bottom had been previously covered with gray paper and moistened with
5 mL of sterile distilled water, to prevent dehydration of the culture medium. Each agar plug was inoculated with a straight
handle loop in both the center and the ends, and covered with a coverslip. The chamber was incubated under previously
selected conditions and observed until the appearance of the fungal colony