Composition of the production medium used was Tyrosine agar
. All the experiments werecarried out in 500 mL Erlenmeyer flasks containing 100 mL of Tyrosine
agar medium. Sterile medium was inoculated with 5% ofinoculum (3.1–4.7
×104 CFU/mL), incubated at 28 ◦C and cultivatedunder agitation at 180 rpm for 7 days. Growth and melanin production
were determined from the samples collected at 12 h intervalsfor which samples (5 mL) were taken from each of three replicatetubes. The cells were harvested by centrifugation at 12,000×g for15 min, at 4 ◦C.