Sugar beet pulp (SBP) is the residue of beet sugar processing and is a promising feedstock for fuel ethanol
production. Response surface methodology was used to investigate the effects of temperature, acid concentration
and solid loading on dilute sulfuric acid pretreatment and enzymatic hydrolysis of SBP. Mass
balances on cellulose, hemicellulose, pectin, and protein were performed and sugar degradation products
such as 5-hydroxymethylfurfural (HMF), furfural and acetic acid were monitored. Scanning electron
microscopy was used to study changes in the physical structure of SBP upon pretreatment. Acid pretreatment
increased the enzymatic digestibility of SBP from 33% (raw) to 93% (treated). Pretreatment at optimum
conditions (temperature = 120 C, acid concentration = 0.66% and solid loading = 6%) resulted in
93% enzymatic hydrolysis yield and 62% total reducing sugar yield. The ethanol yield from pretreated
SBP under the optimum conditions was 0.4 g ethanol/g dry matter in a simultaneous saccharification
and fermentation (SSF) process employing Escherichia coli KO11.
Sugar beet pulp (SBP) is the residue of beet sugar processing and is a promising feedstock for fuel ethanol
production. Response surface methodology was used to investigate the effects of temperature, acid concentration
and solid loading on dilute sulfuric acid pretreatment and enzymatic hydrolysis of SBP. Mass
balances on cellulose, hemicellulose, pectin, and protein were performed and sugar degradation products
such as 5-hydroxymethylfurfural (HMF), furfural and acetic acid were monitored. Scanning electron
microscopy was used to study changes in the physical structure of SBP upon pretreatment. Acid pretreatment
increased the enzymatic digestibility of SBP from 33% (raw) to 93% (treated). Pretreatment at optimum
conditions (temperature = 120 C, acid concentration = 0.66% and solid loading = 6%) resulted in
93% enzymatic hydrolysis yield and 62% total reducing sugar yield. The ethanol yield from pretreated
SBP under the optimum conditions was 0.4 g ethanol/g dry matter in a simultaneous saccharification
and fermentation (SSF) process employing Escherichia coli KO11.
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