Another attempt was to reduce the process of guava leaf
extraction by packing the ground guava leaves in a column
and placing it in the FI manifold. It was found that the guava
leaves packed column-FI system could not be used effectively. The chemical from the leaves only came out at the
edge of each piece, yielding a very small amount of color
reagent in the flow stream. In addition, the active chemical
was used up quickly, resulting in the need to change the column for every analysis, which is not practical.