Tissue sampling: At the end of experiment, rats were sacrificed by decapitation and abdominal incision was immediately done after taking of blood sample for separation of kidney and perirenal, mesentric and epididymal adipose tissues. Kidney was taken and washed by saline, dried by filter paper and weighed 0.5 gm of this tissue then underwent homogenization for tissue biochemical analysis of catalase, MDA and NO.