From the screening experiment, MSL2 showed the highest global cellulase activity (as the FPU), and the second-highest of CMCase activity, therefore it was selected as the best candidate of cellulase-producer of our collection. Cellulase of MSL2 was purified and characterized to find the specific hydrolysis activities with different types of substrates. CMCase fraction of MSL2 was identified to be endoglucanase by using LC-MS/MS analysis