The shelf life of refrigerated fish is usually short due to the chemical and bacteriological activities in fish leading to loss of quality and succeeding spoilage. Current study showed that both shallot fruit and ajwain seed extracts could effectively retard microbial growth, delay chemical deterioration, maintain or improve sensory attributes and extend the shelf life of the semi-fried coated rainbow trout fillets during refrigerated storage. Therefore, natural preservatives such as shallot fruit and ajwain seed extracts can be utilized as a safe preservation method for extending shelf life of chilled rainbow trout products.