Micro propagation is currently the only realistic means of achieving rapid, large-scale production of disease-free quality planting material as seed canes of newly developed varieties in order to speed up the breeding and commercialization process in Sugarcane (Feldmenn et al.,1994 ; Lal and Krishna ,1994 ; Lee ,1987 ; Lorenzo et al., 2001 ; Krisnamurthi and Tlaskal ,1974).