2.2. Enzymes and yeast
GSHE preparation STARGEN 002 was obtained from Genencor
International (USA). STARGEN 002 contains Aspergillus kawachii
amylase expressed in T. reesei and glucoamylase from T. reesei.
As declared by the manufacturer, the preparation activity is 610
glucoamylase units per gram and has an optimal temperature
range of 35–45 ?C. Neutrase 0.8 L (Novozymes, Denmark) is a bacterial
protease from Bacillus amyloliquefaciens, its declared activity
was 0.8 proteolytic units per gram. Ceremix 6X MG
(Novozymes) is a preparation displaying multidirectional substrate