1. Place two sterile Petri plates, lid side up, in front of you. CAUTION: Do not open the lids until you have read the entire procedure
2. Light a Bunsen burner.
3. Obtain a bottle of sterile NA from the 50°C water bath and gently swirl it several times to insure a homogenous mixture.
4. Remove the cap of the bottle.
5. Lightly pass the lip of the bottle through the open flame. The lip of the bottle should already be sterile because it was autoclave, but passing it through the flame eliminates any dust or contaminants you may have introduced while unscrewing the cap.
6. Pour approximately 15 ml of the agar into a plate. Do not remove the Petri dish lid entirely, but open it slightly so that the lid shields the agar from airborne contaminants. Swirl the plate gently so the agar covers the entire bottom of the plate.
7. After one plate is pour, re-flame the lip as before and pour the second plate using the same procedure.
8. Allow the plates to sit undisturbed for 15 to 20 minutes on a flat surface.
9. After the medium has solidified, invert each plate and label it with a marker. Each plate should have your name, date, and type of medium.
1. Place two sterile Petri plates, lid side up, in front of you. CAUTION: Do not open the lids until you have read the entire procedure2. Light a Bunsen burner.3. Obtain a bottle of sterile NA from the 50°C water bath and gently swirl it several times to insure a homogenous mixture.4. Remove the cap of the bottle.5. Lightly pass the lip of the bottle through the open flame. The lip of the bottle should already be sterile because it was autoclave, but passing it through the flame eliminates any dust or contaminants you may have introduced while unscrewing the cap.6. Pour approximately 15 ml of the agar into a plate. Do not remove the Petri dish lid entirely, but open it slightly so that the lid shields the agar from airborne contaminants. Swirl the plate gently so the agar covers the entire bottom of the plate.7. After one plate is pour, re-flame the lip as before and pour the second plate using the same procedure.8. Allow the plates to sit undisturbed for 15 to 20 minutes on a flat surface.9. After the medium has solidified, invert each plate and label it with a marker. Each plate should have your name, date, and type of medium.
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