Layout of the experiments. The three
populations with their parents and the checks
were evaluated for reaction to TLB at 51 days
after planting (DAP). Greenhouse evaluation of
the F2 in April to June of 2009 and field evaluation
of the F2:3 population in October to December of
2009 were not replicated, while an alpha lattice
design (5 blocks of 11 plots/block) of two
replications was used in the field for the F population in April to June of 2010. The F2:3 and
F2:4 families were obtained from 3-5 representative
plants from the previous generation, with equal
portions of seed bulked for evaluation.