Prepared mycelium (approximately 0.5 g) was suspended in extraction buffer (4 M NaCl, 1 M Tris-HCl (pH 8.0), 0.5 M EDTA, 2% PVP) and extracted with phenol: chloroform: isoamyl alcohol (25:24:1) and chloroform/ isoamyl alcohol [24:1].
Prepared mycelium (approximately 0.5 g) was suspended in extraction buffer (4 M NaCl, 1 M Tris-HCl (pH 8.0), 0.5 M EDTA, 2% PVP) and extracted with phenol: chloroform: isoamyl alcohol (25:24:1) and chloroform/ isoamyl alcohol[24:1].