Then, the supernatant was collected into a 20-mL amber glass flask by centrifugation at 3000 rpm for approximately 3 min. The supernatant was passed through a 0.45-m filter and stored at 4◦C before analysis
Then, the supernatant was collected into a 20-mL amber glass flask by centrifugation at 3000 rpm for approximately3 min. The supernatant was passed through a 0.45-m filter and stored at 4◦C before analysis