Expected sized PCR products, 175 bp for primer set I and 245 bp
for primer set II were obtained for all the species studied (except,
for Ahaetulla nasuta, which was not amplified with primer set II).
Thus, the primer set I was 100% effective and the primer set II
showed 91% efficiency in amplifying the targeted region of COI
gene from the samples used. Mini-barcodes were successfully
recovered from all the 11 species studied, in case of primer set I and
for 10 species in case of primer set II.We found 46% and 67% sites as
variable and 29% and 28% sites as parsimony informative in the
barcode data sets obtained from primer sets I and II, respectively.
Mean sequence divergences were 21.9% and 33.4% for sequences
derived from primer sets I and II, respectively (Table 2). The
similarity searches in the public databases enabled identification