It is a truism that a clean sorter is a happy sorter. It is important to make sure
that all fluidics lines be cleaned and/or replaced on a regular basis. If a sort is
aseptic (i.e., one in which cells will be required to be recultured or transplanted),
a sterilization procedure will be needed. In general, this involves running 70%
ethanol through all fluidics lines for 30–60 min before flushing this with distilled
water (30 min) and finally sterile sheath fluid (at least 30 min before commencing
a sort). At all times, fluids pass through a 0.22-