4.4. Strain characterization
For this distinction, the API 50 CHB system was used, which
according to Martínez (2008) is one of the methods most
commonly used for identification at species level. Nigatu, Ahrné,
and Molin (2000) demonstrated that the use of API 50 CH profiles
for taxonomic purpose in most lactobacilli appears reproducible,
but that the specific temperature for the species could affect
the reproduction. The biochemical identification system API 50 CHB
allows identification of the selected strains, although it is advisable
to accompany it with confirmatory evidence.
4.5. GABA
In a human intervention trial, Shimada et al. (2009) used a dietary
GABA-rich algae supplement and Inoue et al. (2003) studied a
new fermented milk containing GABA in patients with mild hypertension.
Both found a significant decrease in blood pressure. The
natural GABA enrichment of food products is commercially a more
interesting approach than its addition as a chemical ingredient and
is particularly relevant to use in the food and biotechnology fields.