3.4. Regulation of MdACS1 and MdERS1 by ethylene
Air treated GS fruit stored at 1 °C that started producing
ethylene (1.38 nl C2H4/g/h) were transferred to 18 °C. The
ethylene production increased to 38.4 nl C2H4/g/h within
24 h (Fig. 4A). However, when some of these fruits were
treated with 1-MCP, the ethylene production decreased to
3.8 nl C2H4/g/h within 24 h of the treatment. Air and 1-MCP
treated fruits were harvested at this stage (S1) and 5 days
later (S2). Transcripts of both MdACS1 and MdERS1 were
abundant in fruits kept in air. The transcript of MdACS1 was
absent in the fruits treated with 1-MCP at S1 which appeared
5 days later, at S2, when the fruit were producing 12.1 nl
C2H4/g/h ethylene. Transcripts of MdERS1 were present at
both stages of 1-MCP treated fruit, though lower than the
controls.
Immature green GS apple fruits kept in air at 18 °C were
exposed to ethylene for 24 h and 48 h and the status of transcript
accumulation of MdACS1 and MdERS1 were monitored
(Fig. 5). Neither air nor ethylene treated fruits produced
ethylene till 11 days post treatment. After 11 days, fruits treated
with ethylene for 24 and 48 h showed an increase in ethylene
production i.e., 0.029 nl C2H4/g/h and 0.341 nl C2H4/g/h
respectively. The control fruit and the fruit treated for 24 h also
did not produce ethylene till 20 days, by which time they were
developmentally competent enough to switch to ethylene
production. Expression of MdACS1 was observed only in fruits
treated with ethylene for 48 h whereas MdERS1 expression was
observed in fruits treated with ethylene for both 24 and 48 h.
3.4. Regulation of MdACS1 and MdERS1 by ethylene
Air treated GS fruit stored at 1 °C that started producing
ethylene (1.38 nl C2H4/g/h) were transferred to 18 °C. The
ethylene production increased to 38.4 nl C2H4/g/h within
24 h (Fig. 4A). However, when some of these fruits were
treated with 1-MCP, the ethylene production decreased to
3.8 nl C2H4/g/h within 24 h of the treatment. Air and 1-MCP
treated fruits were harvested at this stage (S1) and 5 days
later (S2). Transcripts of both MdACS1 and MdERS1 were
abundant in fruits kept in air. The transcript of MdACS1 was
absent in the fruits treated with 1-MCP at S1 which appeared
5 days later, at S2, when the fruit were producing 12.1 nl
C2H4/g/h ethylene. Transcripts of MdERS1 were present at
both stages of 1-MCP treated fruit, though lower than the
controls.
Immature green GS apple fruits kept in air at 18 °C were
exposed to ethylene for 24 h and 48 h and the status of transcript
accumulation of MdACS1 and MdERS1 were monitored
(Fig. 5). Neither air nor ethylene treated fruits produced
ethylene till 11 days post treatment. After 11 days, fruits treated
with ethylene for 24 and 48 h showed an increase in ethylene
production i.e., 0.029 nl C2H4/g/h and 0.341 nl C2H4/g/h
respectively. The control fruit and the fruit treated for 24 h also
did not produce ethylene till 20 days, by which time they were
developmentally competent enough to switch to ethylene
production. Expression of MdACS1 was observed only in fruits
treated with ethylene for 48 h whereas MdERS1 expression was
observed in fruits treated with ethylene for both 24 and 48 h.
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