In summary, we have engineered a single-fiber resonance-enhanced
Raman spectroscope that is uniquely
designed for clinical applications. In this study, we have
shown that this Raman instrument can identify an increase
in the concentrations of BC in cells and additionally denote
the oxidative breakdown of BC after exposure to oxidative
sources as a decrement in the Raman signal of the parent
BC compound. Finally, these results provide proof of
concept that this single-fiber instrument can potentially
measure carotenoids in UADT tissues of patients or any
other tissue accessible endoscopically. These data provide
support for further validation of this technology as a precise
and accurate technology for use during future carotenoid-based
chemopreventive trials