In this study, we selected 18S V7–V9 as the target and amplified
the target sequences using the primers 18S-F1289 (5′-TGGAGYGA
TTTGTCTGGTTDATTCCG-3′; modified from Nishitani et al., 2012) and
18S-R1772 (5′-TCACCTACGGAAACCTTGTTACG-3′; Nishitani et al.,
2012).