The effect of temperature and pressure on the extraction yield of A. Malaccensis by supercritical CO2
was examined in this study as well as the cytotoxic property of the extracted samples. The supercritical
fluid extraction was chosen in this study due to its advantage in producing solvent free samples that
impose measurable level of cytotoxic activity. The extraction parameters were studied to investigate the
extracting condition that gives the highest oil yield from A. Malaccensis. These parameters are;
temperature (40 and 50ºC) pressure (20.7, 27.6 and 34.5 MPa). A fixed matrix particle size (