The milk sampling was carried out during the morning milking. It’s was recorded and weighed to test for bacteria. The experiment for 3 consecutive weekly examinations to test for bacterial infection, somatic cells count and tested for N-Acetyl-β-d-glucosaminidase. Three additional sets of samples were taken from each udder half for analysis as follows. One set was preserved by means of Broad Spectrum Microtabs II and sent to a lab for analysis of the milk composition, fat, protein and lactose in milk. The second set of samples was used to determine Yc and Tc. The third set was defatted and skim milk was used for analysis of casein concentration, whey protein, albumin and peptones proteose for analyze the composition of milk yield with the infection