Collected the midguts, including contents, into a known volume of distilled water and homogenized with a hand-held glass grinder on ice.
Centrifuged the homogenates at 16,000×g at 4 °C for 18 min.
Passed the supernatants through filter paper (pore size, 2.5 μm), transferred to new tubes, and maintained at −20 °C until further use.