The present study aimed to investigate the effect of short-term visual deprivation on the perception of a primary auditory scene analysis cue. Using a harmonicity discrimination task, we demonstrated that 90 min of visual deprivation results in an enhancement of auditory acuity. These results are consistent with recent findings suggesting short-term changes in auditory perception in blindfolded-sighted individuals in a variety of task, including spatial localization and the discrimination of frequency and loudness. It should be noted, however, that not all auditory perceptual tasks previously investigated have revealed such changes. Our results also revealed the transiency of the effect, demonstrating a washout of the enhancement effect 30 min after the removal of the blind-fold