Bacterial auto-aggregation and co-aggregation analysis was
performed according to a protocol by Collado, Surono, Meriluoto,
and Salminen (2007). For auto-aggregation, 107 cells/mL of overnight culturewas centrifuged and three PBS washes were given and suspended in new PBS buffer. Mixture of bacteria was vortexed andincubated at 37C for 4 h. Absorbancewasmeasured at 600 nm and
calculation performed as [1(Atime/Ainitial) 100], where A time
represents the absorbance at 4th h. For autoaggregation DGK-17
was used, and co-aggregation studies were performed DGK-17
with either P. aeruginosa03K711 or K. pneumoniae05K279