According to saccharide profile comparison between starch syrups and pure honeys analysed through
high performance liquid chromatography (HPLC), a characteristic peak was found at 15.25 min retention
time in HPLC chromatogram of syrup, but no peak was observed at the same retention time in chromatogram
of pure honeys. This characteristic peak for syrup was identified as an overlapping peak of oligosaccharides
with more than 5 degree of polymerisation (DP) based on HPLC chromatogram comparison
between starch syrup and a series of standard mono-, di- and oligosaccharides of 3–7 DP. Additionally
syrup content correlated linearly with the height of the characteristic peak of syrup under different slope
in two ranges 2.5–7.5% and 10–100%, respectively. Therefore, the characteristic peak at 15.25 min retention
time can serve as a syrup indicator in HPLC analysis of the adulterated honeys. This new HPLC
method for honey adulteration detection was further applied in an authenticity inspection on more than
100 commercial honeys. In addition to the improved accuracy of honey adulteration detection, the proposed
HPLC method was simple, low cost and easy practice for honey product quality control by government
department considering the popularity of HPLC device and technology.
According to saccharide profile comparison between starch syrups and pure honeys analysed throughhigh performance liquid chromatography (HPLC), a characteristic peak was found at 15.25 min retentiontime in HPLC chromatogram of syrup, but no peak was observed at the same retention time in chromatogramof pure honeys. This characteristic peak for syrup was identified as an overlapping peak of oligosaccharideswith more than 5 degree of polymerisation (DP) based on HPLC chromatogram comparisonbetween starch syrup and a series of standard mono-, di- and oligosaccharides of 3–7 DP. Additionallysyrup content correlated linearly with the height of the characteristic peak of syrup under different slopein two ranges 2.5–7.5% and 10–100%, respectively. Therefore, the characteristic peak at 15.25 min retentiontime can serve as a syrup indicator in HPLC analysis of the adulterated honeys. This new HPLCmethod for honey adulteration detection was further applied in an authenticity inspection on more than100 commercial honeys. In addition to the improved accuracy of honey adulteration detection, the proposedHPLC method was simple, low cost and easy practice for honey product quality control by governmentdepartment considering the popularity of HPLC device and technology.
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