Enzymatic hydrolysis of XRSB was conducted at different
cellulase loadings and the results are shown inFig. 1A. When the
cellulase loading was below 7.5 FPU/g cellulose, the cellulose and
xylan conversions were enhanced with the increased cellulase
loading. However, when the cellulase loading was higher than
7.5 FPU/g cellulose, the cellulose conversion was not significantly
enhanced by the increased cellulase loading. The xylan conversion
was enhanced significantly when 14.9 FPU/g cellulose was
employed. The results indicated that the optimal cellulase loading
was 7.5 FPU/g cellulose. It was reported that when cellulase load-ing was higher than 5 FPU/cellulose, cellulose digestibility was
not enhanced much with the increased cellulase loading when
hydrolyzing COSLIF-pretreated common reeds (Sathitsuksanoh
et al., 2009). However, when dilute acid pretreated biomass was
used as substrate, increased cellulase loading resulted in increased
glucan digestibility (Zhu et al., 2009).