Partially thawed tissues were homogenized with 20mL DI water for 2min, centrifuged for 5min at 12,000×g, and a 0.5mL of surnatant was used for analysis
Partially thawed tissues were homogenized with 20mL DI water for 2min, centrifuged for 5min at 12,000×g, and a 0.5mL of surnatant was used for analysis