In the present study, different substrates were examined with or without addition of supplement for the growth and yield of P. sajor-caju. Mycelial growth is a preliminary step that creates suitable internal conditions for fruiting. Thus, outstanding growth of mycelia is a vital factor in mushroom cultivation (Pokhrel et al., 2009). Mushroom mycelia (vegetative phase) are important in the ecosystem because they are able to biodegrade the substratum and therefore, use the wastes of agricultural products (Manzi et al., 2001). On comparing three substrates used in this study, maize stalk was the best substrate for mycelial extension followed by pea residue and banana leaves. Pea residue with addition of rice bran appeared better substrate after maize stalk supplemented with rice bran. Furthermore, rice bran presented better results as supplement for mycelial growth