To examine whether post-EAMD intervention with carbohydrate supplements could normalize the menstrual cycle and reverse the exercise-induced ovary dysfunction, we treated EAMD rats with glucose and oligosaccharide for 3 weeks. Rats treated with carbohydrate supplements after EAMD showed same menstrual cycles as rats from groups C and R, suggesting that extra energy intakes successfully restored the ovarian function in adult female rats. Furthermore, rats received glucose and oligosaccharide supplements reversed the EAMD-induced mitochondria morphology injuries. We found a slight increase in number of mitochondria and a significant recovery of exercise-induced mitochondria impairment in rats treated with oligosaccharide and glucose, respectively. Our data also showed a significant reduction of serum progesterone level in rats with glucose intake compared to controls, while no differences in 17β-estradiol levels among rats from groups C, R, O, and G. While the reason of failing to restore EAMD-induced attenuation of progesterone in rats received post-EAMD glucose supplement needs further investigation, studies found an insulin sensitivity increases in exercise women,32 which might counter the effect of glucose supplement in EAMD