Extractable polyphenols totals (TEP, mg GAE/100 g) were determined
by the Folin–Ciocalteu method (Obanda & Owuor, 1997).
The extracts (10 and 25 lL of the green and mature fruits respectively)
were mixed with 1 ml of the Folin–Ciocalteu reagent, 2 ml
of the 20% sodium carbonate solution and 2 ml of distilled water.
Following that the samples were agitated and left at rest for at least
30 min in the dark. The readings were made in spectrophotometer
at 700 nm, using the standard curve of gallic acid 98% (0–50 lg).