or HFC diet ( n=24) treatments. Body weight and food intake were recorded weekly. At
the end of week 18, mice were food-deprived for 7 h (7 a.m.–2 p.m.), anesthetized, and
sacrificed by exsanguination via cardiac puncture. Hearts, livers, spleens, kidneys and
visceral fat depots (epididymal, retroperitoneal and mesenteric) were harvested,
rinsed and weighed. Plasma samples were isolated by centrifugation at 3200×g for 15
min. All samples were snap-frozen and stored at − 80 °C until further analysis.