The addition of a small amount of TBA not only has no obvious influences on the HSPC vesicle size and the retention of trapped calcein,but may also result in short freeze-drying cycles. Moreover, freeze-drying of HSPC liposomes from TBA/water co-solvent systems can provide sterile powder for specialized applications.In conjunction with a modified alcohol injection method, this technology may be used to produce dehydrated HSPC liposomes on a large scale.